ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2018.12
2-Monochloropropanediol (2-MCPD), 3-Monochloropropanediol (3- MCPD), and Glycidol in Infant and Adult/Pediatric Nutritional Formula: Gas Chromatographic–Mass Spectrometric (GC–MS) Method
2018 (2023)
First Action
12
AOAC 2023 Online Ch. 50.16.02
Free and ester-bound (bound) analytes are extracted from a powdered test sample by triple heat-ultrasonic-pressure-supported solvent extraction (HUPsSE) using methanol and tert-butyl-methyl-ether (tBME), which cover the polar to the nonpolar range of the analytes. After subsequent application of a liquid/liquid (l/l) extraction, the combined extracts are separated into an aqueous polar fraction containing free 2- and 3-MCPD and a nonpolar fraction containing lipids, bound 2- and 3-MCPD, and bound glycidol. Additionally, this separation step also aids in matrix removal. The free analytes are transferred into an organic phase by l/l extraction ...