ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2018.06
Total Amino Acids in Infant Formulas and Adult Nutritionals: UHPLC– UV
2018 (2023)
First Action
12
AOAC 2023 Online Ch. 50.15.02
Proteins are hydrolyzed in 6 M HC1 for 24 h at 110°C in the presence of phenol, 3-3’-dithiodipropionic acid (DDP), and norvaline (Nva). Phenol (0.1%) is added to prevent halogenation of tyrosine. Nva is added as an internal standard. DDP is added to convert cystine and cysteine to S-2-carboxyethylthiocysteine (XCys) as described by Barkholt and Jensen(1989), and the resulting derivative can be separated from other AAs for quantification ...