ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2017.07
Ethanol in Kombucha, Juices, and Alcohol-Free Beer: ENZYTEC™ Liquid Ethano
2022 (2023)
Final Action
8
AOAC 2023 Online Ch. 30.1.37
The enzymatic reaction requires one enzyme and one co-enzyme only. As can be seen in the reaction below, ethanol is oxidized by the catalytic activity of alcohol dehydrogenase (ADH) in the presence of nicotinamide-adenine dinucleotide (NAD ) to acetaldehyde and NADH/H . NADH formed is stoichiometric with amount of ethanol originally present. NADH produced is measured at 340 nm with a spectrophotometer ...