ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2017.04
cis- and trans-Lutein, cis- and trans-β-Carotene, and cis- and transLycopene in Infant, Pediatric, and Adult Nutritionals: Reversed-Phase HPLC with UV-Visible Detection
2017 (2023)
First Action
10
AOAC 2023 Online Ch. 50.14.02
This reversed-phase HPLC method uses C30 chromatography and UV-Vis spectroscopy to determine cis and trans isomers of lutein, β-carotene, and lycopene in infant, pediatric, and adult nutritionals. Carotenoids are extracted from samples with hexane–methyl tertiary butyl ether (MtBE) (75 + 25) after saponification with a mixture of potassium hydroxide, tetrahydrofuran (THF), and methanol ...