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สหรัฐอเมริกา

AOAC

2017.01

Escherichia coli O157:H7 in Selected Foods: 3M Molecular Detection Assay (MDA) 2 – E. coli O157 (Including H7) Method

2017 (2023)

Final Action

19

AOAC 2023 Online Ch. 17.4.09

The 3M MDA 2 – E. coli O157 (including H7) method is used with the 3M Molecular Detection System (MDS) for the rapid and specific detection of E. coli O157 (including H7) in enriched food samples. The 3M MDA 2 – E. coli O157 (including H7) uses loop-mediated isothermal amplification of unique DNA target sequences with high specificity and sensitivity, combined with bioluminescence to detect the amplification. Presumptive positive results are reported in real-time while negative results are displayed after the assay is completed, 60 min. Samples are enriched in 3M Buffered Peptone Water (BPW; ISO formulation).