ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2016.09
Quantitative Analysis of Aloins and Aloin-Emodin in Aloe Vera Raw Material and Finished Product: High-Performance Liquid Chromatography
2016 (2023)
First Action
7
AOAC 2023 Online Ch. 51.13.01
This test method uses a reversed-phase HPLC system for the separation of aloins A and B and aloe-emodin with an external standard for the quantitation of raw materials and finished products. A dual-wavelength UV detector or photodiode array (PDA) detector can be used. For sample preparation, stepwise liquid–liquid extraction of target analytes (aloins and aloe-modin) was used, followed by the evaporation of extraction media, then reconstitution in solvents that concentrated the target analytes by volume reduction. UV wavelengths were optimized for sensitivity and to reduce interference from sample matrixes ...