ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2014.02
Vitamin B12 (Cyanocobalamin) in Infant Formula and Adult/Pediatric Nutritional Formula: Liquid Chromatography-Ultraviolet Detection
2017 (2023)
Final Action
7
AOAC 2023 Online Ch. 50.2.06
Vitamin B12 is extracted from the sample in a sodium acetate buffer (pH 4) containing cyanide at 100°C for 30 min. Extracts are purified and concentrated with an immunoa}nity column. Vitamin B12 is determined as cyanocobalamin by ultra-high-performance liquid chromatography (UHPLC) or high-performance liquid chromatography (HPLC) with UV detection at 361 nm. Separation takes place on a C18 column using an acetonitrile gradient in water.