ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2013.05
Aflatoxins B1, B2, G1, and G2 in Olive Oil, Peanut Oil, and Sesame Oil: Immunoaxinity Column Cleanup, Postcolumn Derivatization, and LC with Fluorescence Detection
2013 (2023)
9
AOAC 2023 Ch. 49.3.08
A test portion is extracted with methanol–water (55 + 45, v/v). After shaking and centrifuging, the extract is filtered, diluted with water, and applied to an immunoa}nity column (IAC) containing antibodies specific for AFs. After washing with methanol–water (10 + 90, v/v), the AFs are eluted from the column with methanol and quantified by LC with fluorescence detection. For AF post-column derivatization, a photochemical derivatization device or Kobra cell is used.