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AOAC
2012.23
Total Antioxidant Activity: Oxygen Radical Absorbance Capacity (ORAC) Using Fluorescein as the Fluorescence Probe
2012 (2023)
13
AOAC 2023 Ch. 47.2.07
The oxygen radical absorbance capacity (ORAC) method measures the capacity of antioxidants to protect the fluorescent probe from damage by free radicals. In this assay, 2,2′-azobis (2-amidino- propane) dihydrochloride (AAPH) is used as the source for the peroxyl radical, which is generated as a result of the spontaneous decomposition of AAPH at 37 ± 1°C. Fluorescein (FL) is the chosen target probe, whose loss of fluorescence is an indication of the extent of damage from its reaction with the peroxyl radical ...