รายละเอียดเอกสาร

Sample is dissolved in high-salt solution to inhibit protein and fat interactions. The 5′-mononucleotides—uridine 5′-monophosphate (UMP), inosine 5′-monophosphate (IMP), adenosine 5′- monophosphate (AMP), guanosine 5′-monophosphate (GMP), and cytidine 5′-monophosphate (CMP)—are separated from the sample matrix by strong-anion exchange solid-phase extraction (SPE), followed by chromatographic analysis using a C18 stationary phase with gradient elution, UV detection, and quantitation by an internal standard (IS) technique using thymidine 5′-monophosphate (TMP).