ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
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AOAC
2008.04
Hydrastine and Berberine in Goldenseal Raw Materials, Extracts, and Dietary Supplements: High-Performance Liquid Chromatography with UV
2008 (2023)
First Action
3
AOAC 2023 Online Ch. 51.7.01
Hydrastine and berberine are extracted from the matrixes with a mixture of water-acetonitrile-phosphoric acid (70 + 30 + 0.1, v/v/v). The resulting solutions are then subjected to reversed-phase high-performance liquid chromatographic (HPLC) analysis using a C18 column and UV detection. Quantitation is performed using hydrastine and berberine chloride dihydrate external standards and a 5-point calibration curve.