ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2005.05
Shiga Toxin Genes, from Escherichia coli O157:H7, in Selected Foods: Assurance GDS Shiga Toxin Genes (O157)
2012 (2023)
Revised First Action
8
AOAC 2023 Online Ch. 17.4.06A
The Assurance GDS Shiga Toxin Genes (O157) method is a gene-based assay that uses specific primers and proprietary probes directed against highly conserved DNA sequence in the target organism. The method detects stx1 and stx2 genes simultaneously and separately, and uses enrichment in mEHEC broth. After enrichment, populations of target microorganisms are concentrated by using a proprietary concentration device and reagents or an automated concentration instrument ...