ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2005.01
Glucosamine in Raw Materials and Dietary Supplements Containing Glucosamine Sulfate and/or Glucosamine Hydrochloride: HighPerformance Liquid Chromatography with FMOC-Su Derivatization
2008 (2023)
Final Action
13
AOAC 2023 Online Ch. 51.2.01
Glucosamine sulfate/hydrochloride finished products or raw materials are dissolved in water. The glucosamine free base (GFB) is released by adding triethylamine to the solution and derivatized with N-9- fluorenylmethoxycarbonyloxy succinimide (FMOC-Su). The derivative is separated by high-performance liquid chromatography (HPLC) and measured with UV detection. Glucosamine has two natural stereoisomers (α and β), and the interconversion of these two in aqueous solution is not preventable, resulting in two peaks in the chromatogram. The sum of the areas of these two peaks is used to quantify the GFB.