ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2004.11
Identification of Bacillus anthracis from Culture: Gas Chromatographic Analysis of Fatty Acid Methyl Esters (FAMEs)
2004 (2023)
First Action
7
AOAC 2023 Online Ch. 17.14.03
The MIDI method cultures the bacteria, and extracts the fatty acids after saponification in dilute sodium hydroxide-methanol solution followed by derivatization with dilute hydrochloric acid/methanol solution to give the respective FAMEs. The FAMEs are extracted from the aqueous phase by an organic solvent and the resulting solution is analyzed by GC. FAMEs are more volatile than their respective fatty acids and therefore more suitable to GC analysis. The MIDI software automates all analytical operations and uses a pattern recognition algorithm to match the unknown FAME profile to the stored database entries for identification.