ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2002.06
Retinyl Palmitate (Vitamin A) in Fortified Fluid Milk: Liquid Chromatography
2005 (2023)
Final Action
6
AOAC 2023 Online Ch. 45.1.01A
Retinyl esters (vitamin A) from a 2 mL test portion of milk are extracted into hexane containing retinyl acetate as internal standard. The hydrocarbon extract is analyzed without further processing by LC with UV detection (325 nm) using a normal-phase silica column that is equilibrated with mobile phase of hexane−isopropanol (99.85 + 0.15) under controlled temperature. The concentration of retinyl palmitate is calculated by using a relative response factor determined with calibration standards.