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AOAC

2002.04

Amylase-Treated Neutral Detergent Fiber in Feeds: Using Refluxing in Beakers or Crucibles

2005 (2023)

Final Action

15

AOAC 2023 Online Ch. 4.6.04

Fiber in feeds is a nutritionally defined entity that represents the indigestible and slowly digesting fraction of feeds. Neutral detergent (ND) solution and heat-stable α-amylase are used to dissolve easily digested proteins, lipids, sugars, starches, and pectins in feeds, leaving a fibrous residue that is primarily cell wall components in plant materials (cellulose, hemicellulose, and lignin) and indigestible nitrogenous matter in animal products. aNDF is a gravimetric method that best estimates the total insoluble fiber, and is inversely related to digestibility and intake potential of a feed ...