รายละเอียดเอกสาร

Method uses bacterial culture plates of dry media and cold-water-soluble gel. Undiluted or diluted test portions are added to plates at 1.0 mL/plate. Pressure, when applied to plastic spreader placed on overlay lm, spreads the product evenly over a 30 cm growth area. Gelling agent is allowed to solidify, and plates are incubated at 35 ± 1°C for 24 ± 2 h. The plate is incubated at a high temperature (62 ± 2°C) to deactivate heat-labile deoxyribonuclease; then an indicator disk which detects the heat stable enzyme TNase is placed between the top and bottom films of the plate. Plates with disks are incubated at 35 ± 1°C for 1–3 h to identify TNase-positive staphylococci.