ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2000.08
Aflatoxin M1 in Liquid Milk: Immunoaffinity Column by Liquid Chromatography
2004 (2023)
Final Action
6
AOAC 2023 Online Ch. 49.3.07
The test portion is extracted and cleaned up by passing through an immunoa}nity column containing specic antibodies bound onto a solid support. Antibodies selectively bind with any aflatoxin M1 (antigen) contained in the extract, to give an antibody–antigen complex. Other components of matrix are washed o| the column with water. Aflatoxin M 1 from the column is eluted with acetonitrile. After the eluate is concentrated, the amount of aflatoxin M1 is determined by LC with fluorometric detection