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APHA

SMWW 9215

Heterotrophic Plate Count

2023

8

Standard methods for the examination of water and wastewater (543.3 APHA 24th ed. Ref. Treatises&monographs FL2)

The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live, culturable heterotrophs in a sample. Heterotrophs are not taxonomically defined, but are a broad heterogeneous group including, bacteria, yeasts, and molds that require organic carbon for growth.1 Because no single culture technique satisfies all the growth requirements of all heterotrophs in a water sample, the microorganisms that are isolated from a water sample vary based on the culture conditions and inoculation technique used...