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Members of the genus Vibrio are defined as Gram-negative, asporogenous rods that are straight or have a single, rigid curve. They are motile, most having a single polar flagellum when grown in liquid medium. Most produce oxidase and catalase, and ferment glucose without producing gas.65 Vibrio spp. occur naturally in aquatic environments over a wide range of salinities, where they are often the dominant culturable bacteria.63 V. cholerae and its close relative, V. mimicus, are the only Vibrio spp. that can survive and grow in fresh water, and V. cholerae is the only Vibrio frequently transmitted via the fecal-oral route...