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DEFINITION This method is used for the determination of fatty acid esters of 2-chloropropane-1,3-diol (2-MCPD), 3-chloropropane-1,2-diol (3-MCPD) and glycidol in edible oils and fats; see also AOCS Official Methods Cd 29a-13 or Cd 29c-13. Bound glycidol is the sum of all glycidyl derivatives that are cleaved by alkaline-catalyzed alcoholysis. The content of bound glycidol is reported in milligrams per kilogram (mg/kg). Bound 2-MCPD is the sum of all 2-MCPD-derivatives that are cleaved by alkaline-catalyzed alcoholysis. The content of bound 2-MCPD is reported in milligrams per kilogram (mg/kg). Bound 3-MCPD is the sum of all 3-MCPD-derivatives that are cleaved by alkaline-catalyzed alcoholysis. The content of bound 3-MCPD is reported in milligrams per kilogram (mg/kg). SCOPE This method describes a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalyzed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are only present in fats and oils in low to negligible quantities, significant con-tent would increase proportionately the determination of bound analytes. This method is applicable to solid and liquid fats and oils.