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DEFINITION A suitable amount of oil or fat in light petroleum is absorbed on an alumina column; subsequently any benzo(a)pyrene (BaP) present is eluted with light petroleum. Final analysis is carried out by reversed-phase, high-performance, liquid chromatography using a fluorimetric detector (References, 1). SCOPE This method can be used for determining benzo(a)pyrene in crude and refined edible oils and fats with a lowest limit of detection of about 0.1 µg/kg. Interference by other polynuclear aromatic hydrocarbons should not occur if the specified excitation and emission wavelengths are used with the fluorimetric detector.