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DEFINITION Polar compounds are those compounds in oils and fats which are determined by column chromatography under the conditions specified. Frying oils and fats are separated by column chromatography into nonpolar and polar compounds, followed by the elution of the nonpolar compounds. The polar compounds are determined by calculating the difference between the weight of the test sample added to the column and that of the nonpolar fraction eluted. SCOPE This method is for the determination of polar compounds in frying fats. Polar compounds are formed ­during the heating of fats. This method is applicable to animal and vegetable oils and fats. The method serves to assess the deterioration of used frying fats.