เคมี
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOCS
Cd 11c-93
Monoglycerides, Diglycerides, and Triglycerides by Silica Gel Column Chromatography
2017
3
665.0288 AOCS 7th ed. 2017-2019 rev. Ref.
This method determines glyceride mixtures by solid-liquid adsorption chromatography (SLAC), using silica gel as the adsorbent. Triglycerides are eluted with 10% diethyl ether in petroleum ether, diglycerides with 25% diethyl ether in petroleum ether, and monoglycerides with 100% diethyl ether. Free fatty acids present are found predominantly in the triglyceride fraction; the remainder in the diglyceride fraction. Thin-layer chromatography (TLC) is used to verify the purity of the fractions and/or the effectiveness of the separation. This method is applicable to emulsifiers and shortenings.