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These Analytical Guidelines list those methods which may be used to assess the quality of oils and fats used in the production of biodiesel. These Analytical Guidelines are restricted to triglyceride feedstocks for biodiesel manufacture, including vegetable oils (soybean oil, rapeseed oil, palm oil, etc.), animal fats (tallow, lard, etc.), and triglyceride greases (yellow grease, etc.). Critical attributes of the triglycerides are considered in light of the requirements of the most common biodiesel process: the alkaline transesterification of the triglyceride feedstock with methanol to produce fatty acid methyl esters. Only AOCS Official Methods or Recommended Practices are listed in Table 1; however, other procedures are reported in the scientific literature.