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IF 91 (144)

A DNA metabarcoding workflow to identify species in spices and herbs

2023

8

Journal of AOAC INTERNATIONAL, 2023, 106(1), 65–72

Background:

Spices and herbs are food categories regularly cited as highly susceptible to be adulterated. To detect potential adulteration with undeclared species, DNA-based methods are considered the most suitable tools.

Objective:

In this study, the performance of the ready-to-use Thermo Scientific™ NGS Food Authenticity Workflow (Thermo Fisher Scientific)—a commercial DNA metabarcoding approach—is described. The tool was further applied to analyze 272 commercial samples of spices and herbs. ....