ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2006.06
Lactose in Milk: Spectrophotometric Enzymatic Method Using Weight Additions and Path Length Adjustment
2013 (2023)
Final Action
8
AOAC 2023 Online Ch. 33.2.67
Milk, containing negligible endogenous free D-galactose, is diluted, and protein and fat are precipitated and removed by filtration. Lactose in the filtrate is hydrolyzed to D-glucose and D-galactose at pH 4.5 in the presence of β-galactosidase, β-D-Galactose is then oxidized at pH 8.6 by NAD to D-galactonic acid in the presence of β-galactose dehydrogenase ...