ผลิตภัณฑ์อาหาร เครื่องดื่ม อาหารสัตว์
เอกสารมาตรฐาน
สหรัฐอเมริกา
AOAC
2001.06
Total Fumonisins in Corn: Competitive Direct Enzyme-Linked Immunosorbent Assay
2005 (2023)
Final Action
6
AOAC 2023 Online Ch. 49.5.03
This method is a competitive direct enzyme-linked immunosorbent assay (CD-ELISA). Fumonisins (FB1,FB2, and FB3) are extracted from ground test sample with methanol–water (7 + 3, v/v) by blending, then filtering. The extract is diluted 1:80 with methanol–water (1 + 9), and a portion of the diluted extract is mixed with enzyme-labeled fumonisin B1 (conjugate). The mixture is transferred to antibody-coated wells where free toxin and conjugate compete for antibody binding sites ...